This function allows users to keep samples in the sub-datasets (tissues from GTEx or cancer types from TCGA) they selected, and enables users to perform the differential expression in the cell type-level. Up to five sub-datasets are supported.

After clicking the "plot" button, the interactive boxplots grouped by cell type and sub-dataset will be available. The one-way ANOVA function has been dynamically integrated into each sub-group in each boxplot, allowing users to simutenously visualize and compare the gene expression in given cell types.

Parameters

• TCGA Tumor/TCGA Normal/GTEx/Used Expression Datasets: Select cancer types of interest in the "TCGA Tumor", "TCGA Normal" or "GTEx" field and click "add" to build dataset list in the "Used Expression Datasets" field. Also, manual input of cancer types split by comma (e.g. COAD Tumor,READ Tumor) is also acceptable. The differential expression analysis is based on the datasets list.
• Cell Types: Select cell types of interest for analysis.
• Input Gene(s): Input the gene symbol for analysis. You can input the gene in GEPIA in order to verify the correctness of the gene symbol or convert the gene ID or gene alias into the standard gene symbol.