This function allows users to keep samples in the sub-datasets (tissues from GTEx or cancer types from TCGA) they selected, and filter the cell types of interest to visualize and compare their proportions in each bulk sample. Up to five sub-datasets are supported.

After clicking the "plot" button, the interactive boxplots grouped by cell type and sub-dataset will be available. The one-way ANOVA function has been dynamically integrated into each sub-group in each boxplot, allowing users to have a quantitative comparison of the cell proportions.

Parameters

• TCGA Tumor/TCGA Normal/GTEx/Used Expression Datasets: Select cancer types of interest in the "TCGA Tumor", "TCGA Normal" or "GTEx" field and click "add" to build dataset list in the "Used Expression Datasets" field. Also, manual input of cancer types split by comma (e.g. COAD Tumor,READ Tumor) is also acceptable. The cell type proportion analysis is based on the datasets list.
• Cell Types: Select cell types of interest for analysis.
• Normalization: For each sample, normalize the proportions of selected cell types to make the sum equal to 1.